Genetic Analysis and Diagnosis

MF-100 Microfluidic Real-time Fluorescence PCR Instrument


The combination of microfluidic chip and real-time fluorescence quantitative PCR technology has greatly improved the thermal cycling speed, reduced the reagent consumption, has the advantages of high sensitivity and high specificity, can be applied to nucleic acid fast analysis. At present, the company developed MF100-type instrument for scientific research and clinical real-time fluorescent quantitative PCR as a means of genetic testing and expression analysis has been carried out on this platform the bird flu virus, Escherichia coli O157: H7, Listeria monocytogenes And methicillin-resistant Staphylococcus aureus (MRSA) and other common pathogenic microorganisms in rapid detection of experimental studies and early diagnosis of liver cancer-related microRNA expression analysis.

The main application areas:

Clinical disease diagnosis, animal epidemic disease detection, food safety monitoring, genetically modified crops, life sciences research and application of industries: all levels of medical institutions, universities and research institutes, CDC, inspection and Quarantine Bureau, veterinary stations, food companies and dairy products Factory and so on.

At this stage, based on the MF-100 instrument, the company is also focused on integrated micro-total nucleic acid analysis systems for on-site diagnostics (POCT) and on genetic mutations such as single nucleotide polymorphisms (SNPs) Analysis of the development of microfluidic high-resolution melting curve analyzer, with a view to clinical and basic scientific research to provide a more comprehensive molecular diagnostic solution.


Specimen carrier           Microfluidic Chip

Fluorescent label       SYBR Green, LcGreen, EvaGreen, FAM and other fluorescent markers

Reaction system           4-25 μL

Fluorescence excitation wavelength:470nm

Fluorescence emission wavelength: 520 nm

Fluorescence detection method: highly sensitive CCD

Temperature range: 4.0 ℃ -99.0 ℃

Technical Parameters:

L / C speed: ≥ 5.0 ℃ / S

Temperature uniformity: ≤ ± 0.3 ℃

Temperature accuracy: ≤ ± 0.3 ℃

Temperature display accuracy: 0.1 ℃

Sensitivity: single copy

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  • Chinese Academy of Sciences·Yikangda
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  • Tel: 0551-65592125
  • E-mail: ekangda@126.com
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  • Post Code: 230031
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